教員紹介

食・健康科学講座
市川 直樹准教授

Naoki Ichikawa

先生へメッセージを送る

学 位 理学博士
最終学歴 大阪大学大学院 理学研究科(生物化学専攻)
専門分野 生化学・分子生物学
研究テーマ

エネルギー代謝調節系に関する生化学・分子生物学的研究

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生化学関係の基礎科目を担当しています。酵母を実験材料に使って、ミトコンドリアでエネルギーを作り出す酵素の研究をしています。生化学を勉強したい人、酵母に興味のある人、遺伝子操作実験をしてみたい人はぜひいらしてください。

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■研究成果/publications

1. Sun, L. Nakamae, N., and Ichikawa, N. (2015) The region from phenylalanine-28 to lysine-50 of a yeast mitochondrial ATPase inhibitor (IF1) forms an α-helix in solution. J. Bioenerg. Biomembr. 47, 457-465 (doi: 10.1007/s10863-015-9627-7)

2. Ando, C. and Ichikawa, N. (2008) Glutamic acid in the inhibitory site of mitochondrial ATPase inhibitor, IF1, participates in pH sensing in both mammals and yeast. J. Biochem. 144, 547-553 (doi: 10.1093/jb/mvn100)

3. Ichikawa, N., Ando, C., and Fumino, M. (2006) Caenorhabditis elegans MAI-1 protein, which is similar to mitochondrial ATPase inhibitor (IF1), can inhibit yeast F0F1-ATPase but cannot be transported to yeast mitochondria. J. Bioenerg. Biomembr. 38, 93-99 (doi: 10.1007/s10863-006-9009-2)

4. Ichikawa, N., Chisuwa, N., Tanase, M., and Nakamura, M. (2005) Mitochondrial ATP synthase residue βArginine-408, which interacts with the inhibitory site of regulatory protein IF1, is essential for the function of the enzyme. J. Biochem. 138, 201-207 (doi: 10.1093/jb/mvi116)

5. Ichikawa N. and Mizuno, M. (2004) Functional expression of hexahistidine-tagged β-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr. Purif. 37, 97-101 (doi: 10.1016/j.pep.2004.06.002)

6. Ichikawa, N. and Ogura, C. (2003) Overexpression, purification, and characterization of human and bovine mitochondrial ATPase inhibitors: comparison of the properties of mammalian and yeast ATPase inhibitors. J. Bioenerg. Biomembr. 35, 399-407

7. Ichikawa, N., Nakabayashi, K., and Hashimoto, T. (2002) A yeast mitochondrial ATPase inhibitor interacts with three proteins that are easy to dissociate from the mitochondrial inner membrane. J. Biochem. 132, 649-654

8. Ichikawa, N., Karaki, A., Kawabata, M., Ushida, S., Mizushima, M., and Hashimoto, T. (2001) The region from phenylalanine-17 to phenylalanine-28 of a yeast mitochondrial ATPase inhibitor is essential for its ATPase inhibitory activity. J. Biochem. 130, 687-693

9. Ichikawa, N, Fujisaka, R, and Kuribayashi, R. (2000) Requirement for lysine-19 of the yeast mitochondrial ATPase inhibitor for the stability of the inactivated inhibitor-F1Fo complex at higher pH. Biosci. Biotechnol. Biochem. 64, 89-95

10. Ichikawa, N., Ushida, S., Kawabata, M. and Masazumi, Y. (1999) Nucleotide Sequence of cDNA Coding the Mitochondrial Precursor Protein of the ATPase Inhibitor from Humans. Biosci. Biotechnol. Biochem. 63, 2225-2227

11. Ichikawa, N., Fukuda, M., Hashimoto, T., and Tagawa, K. (1998) The carboxyl-terminal region of the yeast ATPase inhibitor is indispensable for the stability of the protein in mitochondria. J. Biochem. 124, 886-891

12. Ichikawa, N., Yoshida, Y., Hashimoto, T., and Tagawa, K. (1996) An intrinsic ATPase inhibitor binds near the active site of yeast mitochondrial F1-ATPase. J. Biochem. 119, 193-199

13. Ichikawa, N., Yoshida, Y., Hashimoto, T., Ogasawara, N., Yoshikawa, H., Imamoto, F., and Tagawa, K. (1990) Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast. J. Biol. Chem. 265, 6274-6278


■主な著書/Books

1. 市川直樹、田川邦夫. 廣川タンパク質化学7 制御タンパク質(インヒビター)「ATPaseインヒビター」 廣川書店 (2002)


■学会活動/Membership in Academic Societies
日本生化学会、他